non malignant human mesothelial cell line met5a  (ATCC)

Journal: Cells

Article Title: Primary and hTERT-Transduced Mesothelioma-Associated Fibroblasts but Not Primary or hTERT-Transduced Mesothelial Cells Stimulate Growth of Human Mesothelioma Cells.

doi: 10.3390/cells12152006

Figure Lengend Snippet: Figure 1. Gene expression profiles of mesothelial cells (NP2, Met5A) and Meso-CAFs (Meso109F, Meso125F) differ from each other, and transduction with hTERT-EGFP (hTERT+) did not induce changes in cell morphologies. (A) Unsupervised clustering of primary mesothelial cells and primary Meso-CAFs. (B) Micrographs of cultured primary NP2 cells (left) and the transduced NP2 derivatives (NP2-hT+) (right). (C) Micrographs of cultured primary Meso-CAFs (left) and the transduced Meso- CAFs derivatives (109F-hT+, 125F-hT+) (right). Scale bar = 100 µm (80×).

Article Snippet: The SV40-immortalized, non-malignant human pleural mesothelial cell line Met5A and both normal primary human lung fibroblasts, MRC-5 and Wi38, were also obtained from ATCC (Rockville, MD, USA) and cultivated in the respective growth medium according to the supplier’s protocol (Met5A in RPMI-1640, MRC-5 in Dulbecco’s Modified Eagle’s Medium (DMEM) (#D5648, Sigma-Aldrich, St. Louis, MO, USA), and Wi38 in Eagle’s minimal essential medium (MEM) (#M5650, Sigma-Aldrich, St. Louis, MO, USA), all supplemented with 10% FBS).

Techniques: Gene Expression, Transduction, Cell Culture